Human P450 Antibodies
CYP450-GP is a leading supplier of specific antibodies to human P450 enzymes. These premium antibodies recognize only their respective P450 antigens of immunization on Western blots/ELISA plus can markedly inhibit metabolism catalyzed by their cognate antigens in intact human liver microsomes. Since cross-reactivity with heterologous P450s has been mostly eliminated, CYP450-GP antibodies provide a powerful tool for metabolic phenotyping studies (see CYP Immunoinhibition Kit below). Unequivocal identification of the P450 enzyme(s) underlying hepatic oxidation of your new NCE/lead compound is now possible.
CYP450-GP P450 antibodies have been produced in rabbits and mice using as antigens:
a) native P450 proteins purified from human liver;
b) recombinant P450 proteins (6x His-labelled at COOH termini) purified from Sf9 insect cells;
c) 20-mer peptides derived from the corresponding full-length P450 amino acid sequences.
Nearly all of our P450 antibodies, including those comprising the CYP Immunoinhibition Kit, exhibit monospecificity on conventional Western blots and/or ELISA*, and can thus be utilized for immunoquantitation studies. Our control (preimmune) antibodies have been obtained from sera taken prior to animal immunization. CYP450-GP antibodies are supplied as purified IgG fractions (either as a lyophylized powder or frozen in solution), and should be stored at -20°C until use. Freeze-thaw cycles should be kept to a minimum. Additional properties are given in the data sheets that accompany the individual products.
*Anti-CYP3A4 IgG (Hu-A005) and anti-CYP1A2 IgG (Hu-A010) are polyspecific in nature, and thus cross-react with other enzymes in the same P450 gene subfamily. For instance, anti-CYP1A2 also recognizes CYP1A1 while anti-CYP3A4 recognizes CYP3A5.
# - Component of CYP Immunoinhibit Kit